Application of immunosignatures to the assessment of Alzheimer's disease
Identifieur interne : 002592 ( Main/Exploration ); précédent : 002591; suivant : 002593Application of immunosignatures to the assessment of Alzheimer's disease
Auteurs : Lucas Restrepo [États-Unis] ; Phillip Stafford [États-Unis] ; D. Mitch Magee [États-Unis] ; Stephen Albert Johnston [États-Unis]Source :
- Annals of Neurology [ 0364-5134 ] ; 2011-08.
Descripteurs français
- KwdFr :
- Analyse sur microréseau (), Animaux, Anticorps (immunologie), Dosage immunologique (), Humains, Maladie d'Alzheimer (diagnostic), Maladie d'Alzheimer (immunologie), Peptides (génétique), Peptides (immunologie), Peptides bêta-amyloïdes (génétique), Peptides bêta-amyloïdes (immunologie), Protéines tau (génétique), Protéines tau (immunologie), Souris, Souris transgéniques, Techniques de diagnostic neurologique.
- MESH :
- diagnostic : Maladie d'Alzheimer.
- génétique : Peptides, Peptides bêta-amyloïdes, Protéines tau.
- immunologie : Anticorps, Maladie d'Alzheimer, Peptides, Peptides bêta-amyloïdes, Protéines tau.
- Analyse sur microréseau, Animaux, Dosage immunologique, Humains, Souris, Souris transgéniques, Techniques de diagnostic neurologique.
English descriptors
- KwdEn :
- Alzheimer Disease (diagnosis), Alzheimer Disease (immunology), Amyloid beta-Peptides (genetics), Amyloid beta-Peptides (immunology), Animals, Antibodies (immunology), Diagnostic Techniques, Neurological, Humans, Immunoassay (methods), Mice, Mice, Transgenic, Microarray Analysis (methods), Peptides (genetics), Peptides (immunology), tau Proteins (genetics), tau Proteins (immunology).
- MESH :
- chemical , genetics : Amyloid beta-Peptides, Peptides, tau Proteins.
- diagnosis : Alzheimer Disease.
- immunology : Alzheimer Disease, Amyloid beta-Peptides, Antibodies, Peptides, tau Proteins.
- methods : Immunoassay, Microarray Analysis.
- Animals, Diagnostic Techniques, Neurological, Humans, Mice, Mice, Transgenic.
Abstract
Objective:: Accurate assessment of Alzheimer's disease (AD), both presymptomatically and at different disease stages, will become increasingly important with the expanding elderly population. There are a number of indications that the immune system is engaged in AD. Here we explore the ability of an antibody‐profiling technology to characterize AD and screen for peptides that may be used for a simple diagnostic test. Methods:: We developed an array‐based system to profile the antibody repertoire of transgenic mice with cerebral amyloidosis (TG) and elderly individuals with or without AD. The array consists of 10,000 random sequence peptides (20‐mers) capable of detecting antibody binding patterns, allowing the identification of peptides that mimic epitopes targeted by a donor's serum. Results:: TG mice exhibited a distinct immunoprofile compared to nontransgenic littermates. Further, we show that dementia patients, including autopsy‐confirmed AD subjects, have distinguishable profiles compared to age‐matched nondemented people. Using antibodies to different forms of Aβ peptide and blocking protocols, we demonstrate that most of this signature is not due to the subject's antibodies raised against Aβ. Interpretation:: We propose that “immunosignaturing” technology may have potential as a diagnostic tool in AD. ANN NEUROL 2011;
Url:
DOI: 10.1002/ana.22405
Affiliations:
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type="ISSN">0364-5134</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0364-5134</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alzheimer Disease (diagnosis)</term><term>Alzheimer Disease (immunology)</term><term>Amyloid beta-Peptides (genetics)</term><term>Amyloid beta-Peptides (immunology)</term><term>Animals</term><term>Antibodies (immunology)</term><term>Diagnostic Techniques, Neurological</term><term>Humans</term><term>Immunoassay (methods)</term><term>Mice</term><term>Mice, Transgenic</term><term>Microarray Analysis (methods)</term><term>Peptides (genetics)</term><term>Peptides (immunology)</term><term>tau Proteins (genetics)</term><term>tau Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse sur microréseau ()</term><term>Animaux</term><term>Anticorps (immunologie)</term><term>Dosage immunologique ()</term><term>Humains</term><term>Maladie d'Alzheimer (diagnostic)</term><term>Maladie d'Alzheimer (immunologie)</term><term>Peptides (génétique)</term><term>Peptides (immunologie)</term><term>Peptides bêta-amyloïdes (génétique)</term><term>Peptides bêta-amyloïdes (immunologie)</term><term>Protéines tau (génétique)</term><term>Protéines tau (immunologie)</term><term>Souris</term><term>Souris transgéniques</term><term>Techniques de diagnostic neurologique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Amyloid beta-Peptides</term><term>Peptides</term><term>tau Proteins</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Alzheimer Disease</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Maladie d'Alzheimer</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Peptides</term><term>Peptides bêta-amyloïdes</term><term>Protéines tau</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps</term><term>Maladie d'Alzheimer</term><term>Peptides</term><term>Peptides bêta-amyloïdes</term><term>Protéines tau</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Alzheimer Disease</term><term>Amyloid beta-Peptides</term><term>Antibodies</term><term>Peptides</term><term>tau Proteins</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Immunoassay</term><term>Microarray Analysis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Diagnostic Techniques, Neurological</term><term>Humans</term><term>Mice</term><term>Mice, Transgenic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse sur microréseau</term><term>Animaux</term><term>Dosage immunologique</term><term>Humains</term><term>Souris</term><term>Souris transgéniques</term><term>Techniques de diagnostic neurologique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Objective:: Accurate assessment of Alzheimer's disease (AD), both presymptomatically and at different disease stages, will become increasingly important with the expanding elderly population. There are a number of indications that the immune system is engaged in AD. Here we explore the ability of an antibody‐profiling technology to characterize AD and screen for peptides that may be used for a simple diagnostic test. Methods:: We developed an array‐based system to profile the antibody repertoire of transgenic mice with cerebral amyloidosis (TG) and elderly individuals with or without AD. The array consists of 10,000 random sequence peptides (20‐mers) capable of detecting antibody binding patterns, allowing the identification of peptides that mimic epitopes targeted by a donor's serum. Results:: TG mice exhibited a distinct immunoprofile compared to nontransgenic littermates. Further, we show that dementia patients, including autopsy‐confirmed AD subjects, have distinguishable profiles compared to age‐matched nondemented people. Using antibodies to different forms of Aβ peptide and blocking protocols, we demonstrate that most of this signature is not due to the subject's antibodies raised against Aβ. Interpretation:: We propose that “immunosignaturing” technology may have potential as a diagnostic tool in AD. ANN NEUROL 2011;</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Arizona</li></region></list><tree><country name="États-Unis"><region name="Arizona"><name sortKey="Restrepo, Lucas" sort="Restrepo, Lucas" uniqKey="Restrepo L" first="Lucas" last="Restrepo">Lucas Restrepo</name></region><name sortKey="Johnston, Stephen Albert" sort="Johnston, Stephen Albert" uniqKey="Johnston S" first="Stephen Albert" last="Johnston">Stephen Albert Johnston</name><name sortKey="Johnston, Stephen Albert" sort="Johnston, Stephen Albert" uniqKey="Johnston S" first="Stephen Albert" last="Johnston">Stephen Albert Johnston</name><name sortKey="Johnston, Stephen Albert" sort="Johnston, Stephen Albert" uniqKey="Johnston S" first="Stephen Albert" last="Johnston">Stephen Albert Johnston</name><name sortKey="Johnston, Stephen Albert" sort="Johnston, Stephen Albert" uniqKey="Johnston S" first="Stephen Albert" last="Johnston">Stephen Albert Johnston</name><name sortKey="Magee, D Mitch" sort="Magee, D Mitch" uniqKey="Magee D" first="D. Mitch" last="Magee">D. Mitch Magee</name><name sortKey="Restrepo, Lucas" sort="Restrepo, Lucas" uniqKey="Restrepo L" first="Lucas" last="Restrepo">Lucas Restrepo</name><name sortKey="Restrepo, Lucas" sort="Restrepo, Lucas" uniqKey="Restrepo L" first="Lucas" last="Restrepo">Lucas Restrepo</name><name sortKey="Stafford, Phillip" sort="Stafford, Phillip" uniqKey="Stafford P" first="Phillip" last="Stafford">Phillip Stafford</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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